Virus Research 229 Seiten 1-8 Elsevier 2/2017.
In recent years there have been several major outbreaks of bovine ephemeral disease in the Middle East,
including Israel. Such occurrences raise the need for quick identification of the viruses responsible for
the outbreaks, in order to rapidly identify the entry of viruses that do not belong to the Middle-East BEFV
lineage. This challenge was met by the development of a high-resolution melt (HRM) assay. The assay
is based on the viral G gene sequence and generation of an algorithm that calculates and evaluates the
GC content of various fragments. The algorithm was designed to scan 50- to 200-base-long segments
in a sliding-window manner, compare and rank them using an Order of Technique of Preference by
Similarity to Ideal Solution (TOPSIS) the technique for order preference by similarity to ideal solution
technique, according to the differences in GC content of homologous fragments. Two fragments were
selected, based on a match to the analysis criteria, in terms of size and GC content. These fragments
were successfully used in the analysis to differentiate between different virus lineages, thus facilitating
assignment of the viruses’ geographical origins. Moreover, the assay could be used for differentiating
infected from vaccinated animales (DIVA). The new algorithm may therefore be useful for development
of improved genotyping studies for other viruses and possibly other microorganisms.