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Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series

Tim Dahmen; Jean-Pierre Baudoin; Andrew R. Lupini; Christian Kübel; Philipp Slusallek; Niels de Jonge
In: Robert Price (Hrsg.). Microscopy and Microanalysis, Vol. 19, No. 7, Pages 1-13, Cambridge University Press, 2014.


In this study, a combined tilt- and focal series is proposed as a new recording scheme for high angle annular dark field (HAADF) scanning transmission electron microscopy (STEM) tomography. Three-dimensional (3D) data was acquired by mechanically tilting the specimen, and recording a through-focal series at each tilt direction. The sample was a whole mount macrophage cell with embedded gold nanoparticles. The tilt focal algebraic reconstruction technique (TF-ART) is introduced as a new algorithm to reconstruct tomograms from such combined tilt- and focal series. The feasibility of TF-ART was demonstrated by 3D reconstruction of the experimental 3D data. The results were compared with a conventional STEM tilt series of a similar sample. The combined tilt- and focal series led to smaller "missing wedge" artifacts, and a higher axial resolution than obtained for the STEM tilt series, thus improving on one of the main issues of tilt series based electron tomography.