Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series

Tim Dahmen, Jean-Pierre Baudoin, Andrew R. Lupini, Christian Kübel, Philipp Slusallek, Niels de Jonge

In: Robert Price (Hrsg.). Microscopy and Microanalysis 19 7 Seiten 1-13 Cambridge University Press 2014.


In this study, a combined tilt- and focal series is proposed as a new recording scheme for high angle annular dark field (HAADF) scanning transmission electron microscopy (STEM) tomography. Three-dimensional (3D) data was acquired by mechanically tilting the specimen, and recording a through-focal series at each tilt direction. The sample was a whole mount macrophage cell with embedded gold nanoparticles. The tilt focal algebraic reconstruction technique (TF-ART) is introduced as a new algorithm to reconstruct tomograms from such combined tilt- and focal series. The feasibility of TF-ART was demonstrated by 3D reconstruction of the experimental 3D data. The results were compared with a conventional STEM tilt series of a similar sample. The combined tilt- and focal series led to smaller "missing wedge" artifacts, and a higher axial resolution than obtained for the STEM tilt series, thus improving on one of the main issues of tilt series based electron tomography.


Deutsches Forschungszentrum für Künstliche Intelligenz
German Research Center for Artificial Intelligence